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谷氨酸门控的氯离子通道(glutamate-gated chloride channels,GluCls)或抑制性谷氨酸受体(inhibitory glutamate receptor,IGluR)是阿维菌素类药剂(avermectins)主要的作用靶标,目前人们对于昆虫的IGluR知之甚少。本实验采用RNA干扰(RNAi)技术对小菜蛾Plutella xylostella IGluR的功能进行了初步研究。结果表明:小菜蛾2龄和3龄幼虫中双链RNA(dsRNA)的最佳注射量分别为50.6nL和71.3nL。实时荧光定量(quantitative real-time PCR,qRT-PCR)检测结果表明,2龄和3龄幼虫在注射dsRNA 36h和24h后IGluR基因的转录后水平分别下降了32.67%和49.30%。幼虫发生RNA干扰后对阿维菌素的敏感性结果显示,注射了IGluR dsRNA的幼虫死亡率显著低于对照。结果说明,小菜蛾IGluR是阿维菌素的潜在靶标之一,为进一步阐明小菜蛾对阿维菌素靶标抗性机理奠定了基础。
Glutamate-gated chloride channels (GluCls) or inhibitory glutamate receptors (IGluRs) are the major targets of avermectins. At present, people Little is known about the IGluR of insects. In this study, RNA interference (RNAi) was used to study the function of Plutella xylostella IGluR. The results showed that the best injection rates of double-stranded RNA (dsRNA) in 2nd and 3rd instar larvae of Plutella xylostella were 50.6nL and 71.3nL, respectively. Quantitative real-time PCR (qRT-PCR) results showed that the levels of IGluR mRNA in the 2nd and 3rd instar larvae decreased by 32.67% and 49.30%, respectively, at 36 and 24 hours after dsRNA injection. Sensitivities to abamectin following larval RNA interference showed that mortality of larvae injected with IGluR dsRNA was significantly lower than that of controls. The results showed that IGluR of Plutella xylostella was one of the potential targets of abamectin, which lays a foundation for further elucidating the mechanism of target resistance to abamectin in Plutella xylostella.