论文部分内容阅读
为了合理利用谷子杂种F1∶2代单株, 以低直链淀粉含量×糯质杂交组合亲本及其F1∶2代植株为材料, 利用形态学和SSR分子标记鉴定F1∶2代植株的真实性.结果表明, 2015 (07) 组合的母本为圆锥形紧穗, 父本为猫爪形松穗;2015 (07)-1至2015 (07)-4等4个F1∶2代单株仅2015 (07)-4为猫爪形、紧穗, SSR标记呈现父母本的互补型条带, 为真杂种, 其余3个单株株形、穗型上与母本相同, SSR带型与母本相同为假杂种.2015 (13) 组合双亲均为圆锥形穗, 茎、叶深绿, F1∶2代植株形态上不易识别;2015 (13) 组合从40对SSR引物中筛选到双亲间多态性较好的引物17对, 采用这17对引物对2015 (13)-1至2015 (13)-13等13个F1∶2代单株基因组DNA扩增检测表明, 仅2015 (13)-6和2015 (13)-9的SSR带型与母本相同为假杂种, 其余11个单株均为互补型条带, 为真杂种.组合2015 (07) 和2015 (13) 杂种F1∶2代当选单株的真实性频率分别为25.0％和84.6％.本研究通过田间观测结合SSR分子标记, 对谷子F1代当选单株的真实性进行鉴定, 为F2∶3代群体种植及其单株选择提供实验依据.“,”In order to rationally use millet hybrid F1:2 plants, parents combined by low amylose content×waxy millet and their hybrid F1:2 plants were used as materials to identify the truth of F1:2 plants by morphology and SSR molecular markers. The results showed that female parent of 2015 (07) combination was conical tight spike, while the male parent was cat claw loose spike. 2015 (07)-4 was true hybrid associated with cat claw tight spike, and complementary bands of their parents in SSR markers, and the other three plants were false hybrid with conical tight spike, same as the mother belt in SSR markers. Parents of 2015 (13) combination had conical spikes, dark green stem and leaves, and F1:2 was undistinguishable in morphology. 17 pairs of better polymorphism primers between parents were screened from 40 pairs of SSR primers in 2015 (13) combination. 13 F1:2 plants from 2015 (13)-1 to2015 (13)-13 were detected for PCR amplification using these 17 paris of primers, the result of which showed that only 2015 (13)-6 and 2015 (13)-9 were false hybrids, and their SSR belt types were same as the mother belt. The other11 plants were true hybrids owing to their SSR belts were complementary bands of their parents in SSR markers. F1:2 plants fidelity frequency of the combination of 2015 (07) and 2015 (13) were 25. 0％ and 84. 6％, respectively. In this study, the authenticity of the F1 generation of millet was identified by field observation combined with SSR molecular markers, which provided experimental evidence for F2:3 generation population selection and individual plant selection.