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目的:探讨复方楂金颗粒(CZJG)中试产品对高脂饲料诱导的非酒精性脂肪肝炎大鼠脂质代谢及肝细胞超微结构的影响。方法:SD大鼠60只,随机分为正常组、模型组、易善复组(61.6 mg/kg)及复方楂金颗粒高、中、低剂量组(3.50、1.75、0.88 g/kg),每组10只。以高脂饲料喂养造模,给药12周后,处死所有大鼠,测定大鼠肝组织匀浆上清液TC、TG、FFA的含量;ELISA法检测大鼠肝匀浆中SOD、MDA、GSH-Px、TAOC的活性;透射电子显微镜观察肝细胞的超微结构。结果:与模型组比较,CZJG各剂量组大鼠肝组织匀浆TC含量均显著降低(P<0.05),中剂量组TG含量、高剂量组FFA含量显著降低(P<0.05);低剂量组SOD水平显著升高(P<0.01),高、低剂量组MDA水平显著降低(P<0.05;P<0.01)。电镜下可见模型组肝细胞超微结构异常,肝细胞胞浆脂滴聚积,线粒体嵴变少缩短,细胞核部分呈现固缩;CZJG高剂量组肝细胞胞浆可见大量脂滴,线粒体多数嵴清晰可见,细胞核呈圆形,无明显异常。结论:CZJG中试产品可有效调节NASH实验大鼠肝脏脂质代谢及抗脂质过氧化水平,其作用可能与改善线粒体代谢等有关。
Objective: To investigate the effect of CZJG pilot product on lipid metabolism and hepatocyte ultrastructure induced by high fat diet in nonalcoholic steatohepatitis rats. Methods: Sixty Sprague-Dawley rats were randomly divided into normal group, model group, YiShenFu group (61.6 mg / kg) and high, medium and low doses of compound hawthorn gold granules (3.50,1.75,0.88 g / kg) Each group of 10. After 12 weeks of administration, all the rats were sacrificed, the contents of TC, TG and FFA in the liver homogenate supernatant of rats were determined. The levels of SOD, MDA, GSH-Px and TAOC. The ultrastructure of hepatocytes was observed by transmission electron microscope. Results: Compared with the model group, the content of TC in the liver homogenate of CZJG group was significantly decreased (P <0.05), the content of TG in medium dose group and the content of FFA in high dose group were significantly decreased (P <0.05) (P <0.01). The levels of MDA in high and low dose groups were significantly decreased (P <0.05; P <0.01). Electron microscopy showed that the ultrastructure of liver cells in the model group was abnormal. The lipid droplets accumulated in the cytoplasm of the model group, the number of mitochondrial cristae decreased and the number of lipid droplets in the cytoplasm of CZJG high-dose group was visible. Most mitochondria were clearly visible , The nucleus was round, no obvious abnormalities. Conclusion: The CZJG pilot product can effectively regulate liver lipid metabolism and lipid peroxidation levels in NASH rats, which may be related to the improvement of mitochondrial metabolism.