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Ninety wild strains of Agaricus collected in China,including 44 strains preliminarily identified as Agaricus bisporus by isozyme electrophoresis,were studied using SRAP and ISSR techniques.Eighteen specific SRAP bands and 12 specific ISSR bands were scored,and a cluster dendrogram was constructed.The 90 wild strains of Agaricus were separated into two major groups,the first consisting of wild strains of A.bisporus and the second consisting of other Agaricus strains.Forty-one wild strains of A.bisporus collected in Sichuan and Tibet formed four groups according to the geographical location of the collection area.White A.bisporus strains from Xinjiang and Tibet(Xinjiang Wild,AgX04 and AgX042)exhibited unique band patterns resulting in lower similarity coefficient values(10%～13%)when compared with brown or light-brown A.bisporus strains.
Ninety wild strains of Agaricus collected in China, including 44 preliminarily identified as Agaricus bisporus by isozyme electrophoresis, were studied using SRAP and ISSR techniques. Eighteen specific SRAP bands and 12 specific ISSR bands were scored, and a cluster dendrogram was constructed. 90 wild of of Agaricus were separated into two major groups, the first consisting of wild strains of A. bisporus and the second consisting of other Agaricus strains. Forty-one wild strains of A. bisporus collected in Sichuan and Tibet formed four groups according to the geographical location of the collection area. White A.bisporus strains from Xinjiang and Tibet (Xinjiang Wild, AgX04 and AgX042) demonstrated unique band patterns resulting in lower similarity coefficient values (10% ~ 13%) when compared with brown or light-brown A Bisporus.