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目的比较薄层色谱法（ＴＬＣ）、改良纸薄层色谱法（ＬＴＤ）和一步法（ＳｕｒｅｓｔｅｐＴＭ）对海洛因依赖者尿吗啡定性检测的敏感性。方法３０例海洛因依赖者脱毒过程中逐日尿液标本采用该三种方法进行检测，并以３０名健康者尿液标本作对照。结果３０名健康者尿液标本吗啡定性检测三种方法均呈阴性。ＴＬＣ法戒断毒品３～４天，ＬＴＤ法４～６天，ＳｕｒｅｓｔｅｐＴＭ法６～１５天尿毒品检测转为阴性，灵敏度ＳｕｒｅｓｔｅｐＴＭ０．２～０．３μｇ／ｍｌ、ＬＴＤ０．４～０．５μｇ／ｍｌ、ＴＬＣ０．６～０．７μｇ／ｍｌ。结论ＴＬＣ法准确性高、经济、易开展，但灵敏度差、检测费时。ＬＴＤ法准确性好、灵敏度较高、检测时间短，但成本高。ＳｕｒｅｓｔｅｐＴＭ是一种免疫色谱法，一步操作可得结果，灵敏度高，但准确性差，主要用于初筛。
Objective To compare the sensitivity of thin layer chromatography (TLC), modified paper thin layer chromatography (LTD) and one-step (SurestepTM) to the qualitative detection of urinary morphine in heroin dependent patients. Methods Thirty urine samples of daily heroin addicts during detoxification were tested by the three methods. Urine specimens from 30 healthy volunteers were used as controls. Results 30 healthy subjects urine samples morphine qualitative detection of three methods were negative. TLC method to abstain from drug 3 to 4 days, LTD method 4 to 6 days, SurestepTM method 6 to 15 days urine test turned negative, the sensitivity SurestepTM0.2 ~ 0.3μg / ml, LTD0.4 ~ 0.5μg / ml , TLC 0.6 ~ 0.7μg / ml. Conclusion TLC method is accurate, economical and easy to carry out, but its sensitivity is poor and testing is time-consuming. LTD Law accuracy, high sensitivity, detection time is short, but the high cost. SurestepTM is an immunochromatographic, one-step procedure that produces results with high sensitivity but poor accuracy, primarily for primary screening.