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运用抗ＣＤ１ａ单克隆抗体免疫组化方法检测了１０例正常皮肤毛囊皮脂腺单位中朗格汉斯细胞（ＬＣ）的定位和定量情况。结果显示ＬＣ主要集中于毛囊上中部：如漏斗部平均１９８７个／毛囊（范围８～５４个）、皮脂腺导管和腺上皮中平均１６３５个（范围７～３４个），其分布密度类似于表皮；相反，在毛囊下部ＬＣ数量明显减少，平均４１６个／毛囊，且主要见于其上段的隆突部附近；毛球部几乎见不到ＣＤ１ａ阳性ＬＣ。这种分布模式与红斑狼疮、扁平苔藓和斑秃等疾病的炎症浸润模式和临床后果具有一致性；进一步支持ＬＣ可能是这些Ｔ细胞介导的炎症病变的激发相关细胞和／或靶细胞。
The localization and quantitation of Langerhans cells (LCs) in sebaceous units of 10 normal skin follicles were detected by immunohistochemistry with anti-CD1a monoclonal antibody. The results showed that LC mainly concentrated in the middle of the hair follicles: an average of 1987 per hair follicle (range of 8 to 54) in the funnel, an average of 1635 (range of 7 to 34) in the sebaceous duct and glandular epithelium, the distribution density Similar to the epidermis; on the contrary, the number of LCs in the lower part of the hair follicles decreased significantly, with an average of 4.16 / hair follicles, mainly in the vicinity of the bulge in the upper part of the hair follicles; CD1a-positive LCs were hardly seen in the hair bulb parts. This pattern of distribution is consistent with the pattern of inflammatory infiltrates and clinical consequences of diseases such as lupus erythematosus, lichen planus and alopecia areata; further support for LC may be an apoptosis-related cell and / or a target cell for these T cell-mediated inflammatory pathologies.