本文考察了邻苯二甲醛荧光检测蛋白质的方法中各种因素的影响,测定了其检测的线性范围,并对其灵敏度与紫外检测法进行了比较。由于此法具有反应快、检测灵敏度较高和线性范围较宽等优点,可考虑用为柱后衍生的高效液相色谱法以检测分离的蛋白质。 In this paper, we investigated the influence of various factors in the method of fluorescence detection of orthophthalaldehyde, measured the linear range of its detection, and compared its sensitivity with UV detection. Because of its fast response, high detection sensitivity, and wide linear range, high performance liquid chromatography with column derivatization can be considered for the detection of isolated proteins.