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目的 研究哮喘病人免疫球蛋白E高亲和受体 β链基因多态性。方法 提取 4 0例哮喘和 3 6名健康人外周血DNA ,PCR扩增免疫球蛋白E高亲和受体 β链基因 ,分析第二个内含子内限制性内切酶Rsa 1酶切位点的多态性。结果 免疫球蛋白E高亲和受体 β链等位基因A纯合子、等位基因B纯合子和杂合子的分布频率在哮喘组和健康对照组间无显著性差别。结论 免疫球蛋白E高亲和受体 β链基因的多态性与哮喘的发生无相关关系。
Objective To study the polymorphism of immunoglobulin E high affinity receptor β chain gene in patients with asthma. Methods Peripheral blood DNA from 40 asthmatic patients and 36 healthy controls were extracted and the immunoglobulin E high affinity receptor β chain gene was amplified by PCR. The second intron restriction endonuclease Rsa 1 Point polymorphism. Results The frequencies of immunoglobulin E high affinity receptor β chain allele A homozygote, allele B homozygote and heterozygote had no significant difference between the asthma group and the healthy control group. Conclusion The polymorphism of immunoglobulin E high affinity receptor beta chain gene has no correlation with the occurrence of asthma.