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目的探讨了肝纤维化的发生与慢性肝病患者单个核细胞(PBMC)及血浆中转化生长因子β1(TGF-β1)的关系。方法采用荧光定量逆转录聚合酶链反应(FQ-RT-PCR)方法检测慢性肝病患者PBMC中TGF-β1 mRNA含量,采用ELISA方法检测血浆中TGF-β1含量,采用放射免疫方法检测HA、LN、CIV、PCⅢ含量。同时对检测结果进行了对比分析,以探讨各检测指标的相关性。结果慢性乙型肝炎中重度患者及患有肝炎的肝纤维化患者PBMC中TGF-β1含量及血浆TGF-β1水平均显著高于正常水平,且与HA,LN,CIV,PCⅢ四项肝纤维化诊断指标呈正相关。结论慢性肝病患者PBMC及血浆中TGF-β1含量水平可以作为肝纤维化的诊断指标,但能否作为早期诊断的敏感指标,仍需要临床大量试验证实。
Objective To investigate the relationship between the occurrence of hepatic fibrosis and mononuclear cells (PBMC) and transforming growth factor β1 (TGF-β1) in patients with chronic liver disease. Methods The level of TGF-β1 mRNA in PBMC of patients with chronic liver disease was detected by fluorescence quantitative reverse transcription polymerase chain reaction (FQ-RT-PCR). The content of TGF-β1 in plasma was detected by ELISA. The levels of HA, LN, CIV, PCⅢ content. At the same time, the test results were compared and analyzed in order to explore the correlation of each test index. Results The levels of TGF-β1 and TGF-β1 in PBMC of patients with chronic hepatitis B and those with hepatic fibrosis were significantly higher than those of normal, and were significantly correlated with those of liver fibrosis with HA, LN, CIV and PCⅢ Diagnostic indicators were positively correlated. Conclusion The levels of TGF-β1 in peripheral blood mononuclear cells and plasma in patients with chronic liver disease can be used as a diagnostic index for liver fibrosis. However, whether it can be used as a sensitive indicator for early diagnosis still needs clinical confirmation.