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目的 研究人类骨肉瘤细胞血管形成因子的作用及其生物活性。方法 通过对人类骨肉瘤组织及培养中的骨肉瘤细胞进行盐析,以及对培养中收集的大量条件培养基进行超滤、层析、透析等截留分子量8 000～10 000dalt的蛋白质纯化,获取盐析分段及19个层析分段。以人类脐静脉内皮细胞增殖反应为实验对象,用作检验该盐析和层析分段的生物活性。结果 40％的盐析分段及层析中的20ml～30ml分段具有显著的促进内皮细胞增殖的生物学效应。结论 据此表明人类骨肉瘤细胞能够合成并分泌血管形成因子,其分子量约在8 000～10 000dalt之间。
Objective To study the effect and biological activity of angiogenic factors in human osteosarcoma cells. Methods The osteosarcoma cells in human osteosarcoma tissue and culture were salted out and purified by ultrafiltration, chromatography, dialysis and other proteins with molecular weight cut-off of 8 000 ~ 10 000 dalt, and the salt Segmentation and 19 Chromatographic Segments. The human umbilical vein endothelial cell proliferation reaction was used as a test object to test the biological activity of the salting out and chromatography. Results 40% of the salting-out sections and 20ml-30ml sections of the chromatography had significant biological effects of promoting endothelial cell proliferation. Conclusions This indicates that human osteosarcoma cells can synthesize and secrete angiogenic factors with molecular weights of about 8 000 -10 000 dalt.