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目的 研究体外液压冲击伤后大鼠神经细胞内游离钙和 pH值的变化及其药物的保护作用。 方法 培养新生乳鼠的大脑皮层神经细胞 ,给予 2 5kPa ,2 0ms的液压冲击伤 ,通过激光扫描共聚焦显微镜检测伤后单个神经细胞内游离 [Ca2 +]i和pH值的变化 ,并分别给予尼莫地平和D AP 5 ,观察药物对上述变化的影响。结果 伤后细胞内 [Ca2 +]i迅速升高 ,持续 12h达高峰 ,随后逐渐下降 ,48h接近正常 ;pH值下降较慢 ,于 12h达低谷 ,48h未恢复正常。尼莫地平和D AP 5均可明显抑制细胞内 [Ca2 +]i的升高和pH值的下降。结论 可根据液压冲击伤后神经细胞内游离 [Ca2 +]i及 pH值的变化规律指导用药
Objective To study the changes of intracellular free calcium and pH value and the protective effect of the drug on rat neurons after hydraulic shock injury. Methods Neonatal neonatal rat cerebral cortical neurons were cultured and subjected to 25kPa and 2ms hydraulic impingement injury. The changes of free Ca2 + i and pH value in injured individual neurons were detected by laser scanning confocal microscopy and were respectively given Nimodipine and D AP 5, observed the impact of drugs on these changes. Results The intracellular [Ca2 +] i increased rapidly and reached the peak at 12h after injury, and then gradually decreased at 48h, which was close to normal at 48h. The pH value decreased slowly, reached a low level at 12h and did not return to normal after 48h. Both nimodipine and DAP 5 significantly inhibited intracellular [Ca 2+] i elevation and pH drop. Conclusion According to the changes of free [Ca2 +] i and pH value in nerve cells after hydraulic shock injury,