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目的:观察新生大鼠缺氧缺血(H I)后不同脑区神经元变性的动态变化,探讨其与星形胶质细胞反应的关系。方法:采用F luoro-Jade B(FJB)染色法及免疫组化法分别观察变性神经元荧光染色强度及胶质纤维酸性蛋白(GFAP)表达的动态变化。结果:正常新生大鼠各脑区内均未见FJB阳性细胞,GFAP呈弱表达。H I后1 d即可见FJB阳性细胞,3 d阳性细胞增多,5 d达高峰,以纹状体受累最重,皮质次之,海马各区易损顺序为CA1、CA4、CA2、CA3、齿状回。GFAP表达的强度与FJB阳性细胞数密切相关。结论:新生大鼠H I后脑易损部位为纹状体、皮质、海马,FJB染色是一种标记变性神经元的有效方法,反应性胶质细胞增生是应答神经元损伤的结果。
OBJECTIVE: To observe the dynamic changes of neuron degeneration in different brain regions of neonatal rats after hypoxia-ischemia (H I), and to explore its relationship with astrocyte reaction. Methods: F luoro-Jade B (FJB) staining and immunohistochemistry were used to observe the dynamic changes of fluorescence intensity and glial fibrillary acidic protein (GFAP) expression in degenerative neurons. Results: FJB positive cells were not found in all brain regions of normal neonatal rats, and GFAP was weakly expressed. The FJB positive cells were observed on the first day after HI, the number of positive cells increased on the 3rd day, and reached the peak on the 5th day. The strongest striatum was involved in the cortex, followed by the cortex. The order of fragility was hippocampus CA1, CA4, CA2, CA3, dentate gyrus . The intensity of GFAP expression is closely related to the number of FJB positive cells. CONCLUSION: The fragile striatum, cortex, hippocampus and FJB staining of neonatal rat H I posterior brain are an effective method to label degenerative neurons. Reactive glial cell proliferation is the result of neuronal damage.