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目的:研究HPV-18病毒E2、E6与Brd4对宫颈癌及癌前病变的意义。方法:选择2009年3月至2011年3月我院接诊的17例宫颈炎患者,19例CINⅠ级(轻度非典型增生)患者,14例CINⅡ级(中度非典型增生)患者,15例CINⅢ级(重度非典型增生及原位癌)患者,19例宫颈癌患者。分别采用R T-PC R对各组的E2 mR N A、E6 mR N A阳性表达情况进行测定,采用蛋白印迹(Western Blot)法对各组患者的E2蛋白与Brd4表达情况进行测定。从而分析HPV-18病毒E2、E6与Brd4对宫颈癌及癌前病变的临床意义。结果:各项检测后,发现宫颈癌组患者的E2 mR N A阳性表达率明显低于宫颈炎组、CIN组(P<0.05);宫颈癌组患者的E6 mR N A阳性表达率明显高于宫颈炎组、CIN组(P<0.05)。HPV-18病毒E2 mR N A与E6 mR N A阳性表达情况呈负相关(P<0.05)。宫颈癌组患者的E2蛋白阳性率明显低于宫颈炎组、CIN组(P<0.05);宫颈癌组患者的Brd4蛋白阳性率明显低于宫颈炎组、CIN组(P<0.05)。结论:HPV-18病毒E2、E6与Brd4对宫颈癌及癌前病变的检测有重要意义,可用于对于宫颈癌演变过程的监控。
Objective: To study the significance of HPV-18 E2, E6 and Brd4 on cervical cancer and precancerous lesions. Methods: From March 2009 to March 2011, 17 cases of cervicitis treated in our hospital, 19 patients with CINⅠ (mild atypical hyperplasia), 14 patients with CINⅡ (moderate atypical hyperplasia), 15 Cases of CIN Ⅲ grade (severe atypical hyperplasia and carcinoma in situ), 19 cases of cervical cancer patients. The positive expression of E2 mR N A and E6 mR N A in each group was determined by R T-PC R respectively. The expressions of E2 protein and Brd4 in each group were determined by Western Blot. To analyze the clinical significance of HPV-18 E2, E6 and Brd4 on cervical cancer and precancerous lesions. Results: The positive rate of E2 mR NA in cervical cancer patients was significantly lower than that in cervicitis patients and CIN patients (P <0.05). The positive rate of E6 mR NA in patients with cervical cancer was significantly higher than that in patients with cervicitis Group, CIN group (P <0.05). There was a negative correlation between E2 mR N A and E6 mR N A expression in HPV-18 virus (P <0.05). The positive rate of E2 protein in cervical cancer patients was significantly lower than that in cervicitis patients and CIN patients (P <0.05). The positive rate of Brd4 protein in cervical cancer patients was significantly lower than that in cervicitis patients and CIN patients (P <0.05). Conclusion: The detection of HPV-18 E2, E6 and Brd4 on cervical cancer and precancerous lesions is of great significance and can be used to monitor the evolution of cervical cancer.