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目的初步分析重庆地区0～6岁儿童血清人偏肺病毒(hMPV)中和活性及与 hMPVIgG 抗体水平的关系,为进一步大样本的血清流行病学研究奠定基础。方法 0～3岁儿童血清来自因外科疾患住院的儿童,3～6岁儿童血清来自托幼儿童体检血清。采用微量中和实验检测上述血清中 hMPV 中和活性,分析血清中和活性水平及其与 hMPV IgG 抗体水平的相关性。结果在抽取的50份血清标本中,此前通过间接酶联免疫吸附试验检测为阴性的8份标本均未显示中和活性,而42份 hMPV IgG 抗体阳性血清标本中和活性均显阳性,平均中和滴度为1:129。其中0～5个月组中和滴度均值1:160,6～11、12～23、24～35个月和3～6岁组中和滴度均值分别为1:58.9、1:114.9、1:172.8、1:160。血清中抗 hMPV IgG 水平和中和活性具有显著相关性,r=0.668,但 hMPV IgG 水平与中和活性并不平行。结论 0～6岁儿童血清中多具 hMPV 中和活性,但其是否足以预防不同亚型hMPV 感染尚待进一步研究。
Objective To analyze the relationship between hMPV neutralizing activity and hMPVIgG antibody level in children aged 0 ~ 6 years in Chongqing, and to lay a foundation for the further study on serum epidemiology of large samples. Methods Serum from 0 to 3 years old children from hospitalized patients with surgical conditions, 3 to 6-year-old children serum from kindergarten children examination. The neutralizing activity of hMPV in the above serum was detected by micro-neutralization test, and the correlation between serum neutralizing activity and hMPV IgG antibody level was analyzed. RESULTS: Of the 50 serum samples collected, none of the 8 specimens previously tested negative by indirect ELISA showed neutralizing activity, while 42 samples of hMPV IgG positive serum showed positive neutralizing activity And titers of 1: 129. The average titers of neutralization in 0-5 months group were 1: 160,6 to 11,12 to 23,24 to 35 months and the average titers of neutralization in 3 to 6 years group were 1: 58.9 and 1: 114.9, respectively, 1: 172.8,1: 160. There was a significant correlation between anti-hMPV IgG levels and neutralizing activity in serum, r = 0.668, but hMPV IgG levels were not paralleled by neutralizing activity. Conclusions There is more hMPV neutralizing activity in the serum of children aged 0-6 years. However, it is still not yet studied whether hMPV is sufficient to prevent hMPV infection in different subtypes.