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目的探讨FasL基因诱导胶质瘤细胞凋亡的作用。方法以脂质体将FasL基因转染NIH3T3纤维母细胞,采用免疫组化染色法和RT-PCR法检测NIH3T3/FasL细胞中目的基因的表达和转录,Hochest33342荧光染色法和TUNEL法检测共培养条件下观察其对胶质瘤细胞BT325诱导凋亡的作用。结果NIH3T3/FasL细胞能有效表达FasL,与NIH3T3细胞(对照组)相比,有显著诱导BT325细胞凋亡的作用(P﹤0.05)。结论转染了FasL基因的NIH3T3细胞可诱导胶质瘤细胞凋亡。
Objective To investigate the role of FasL gene in inducing glioma cell apoptosis. Methods FasL gene was transfected into NIH3T3 fibroblasts by liposome. The expression and transcription of target gene in NIH3T3 / FasL cells were detected by immunohistochemical staining and RT-PCR. Hochest33342 fluorescence staining and TUNEL assay were used to detect the co-culture conditions Observed under the glioma cell BT325 induced apoptosis. Results NIH3T3 / FasL cells could effectively express FasL, which significantly induced the apoptosis of BT325 cells compared with NIH3T3 cells (control group) (P <0.05). Conclusion NIH3T3 cells transfected with FasL gene can induce glioma cell apoptosis.