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该研究通过制备大鼠外翻肠囊模型,并对其转运活性进行了确认,以秦艽中的马钱苷酸、当药苦苷、龙胆苦苷、以及当药苷为检测指标,用HPLC对经肠囊主动转运吸收后被吸收进入浆膜层的秦艽提取物中的主要成分群进行了分析,探讨了中药材主要吸收成分群的研究方法。结果表明所采用的大鼠外翻肠囊模型,在实验45 min内,组织结构完整,转运活性良好;HPLC的专属性和线性关系良好,精密度的RSD<5.5%;样品的萃取回收率均在90%以上;在室温条件下,样品的稳定性良好。秦艽提取物经肠囊主动转运后,在浆膜液样品中,检测到了8种主要成分;提取物的浓度对主要成分的吸收具有极其显著影响,而不同肠段对主要成分吸收的影响没有显著差异。由此可见,大鼠外翻肠囊技术可用于某些中药材的吸收成分群的研究,是一种探讨中药材活性成分的有效方法。
In this study, the rat valgus enteral sac model was prepared and its transport activity was confirmed. In this study, Flunarizine, Pinamargin, gentiopicroside, and when the glycosides were used as detection index, HPLC The main constituents in the extract of Gentiana macrophylla which were absorbed by the enteric sac and transported into the serosal layer were analyzed, and the research methods of the main absorption components of the traditional Chinese medicinal materials were discussed. The results showed that the model of rat everted gut capsule was intact and had good transport activity within 45 min. The HPLC specificity and linearity were good, and the RSD of precision was <5.5%. The extraction recovery More than 90%; at room temperature, the stability of the sample is good. After the active extract of Gentiana macrophylla was translocated through the gut, eight main components were detected in the serous fluid sample. The concentration of the extract had an extremely significant effect on the absorption of the main components, while the effect of different intestinal segments on the absorption of the main components was not significant difference. Thus, the rat everted gut sac technology can be used for some Chinese herbal medicine absorption component group is an effective method to explore the active ingredients of Chinese herbal medicine.