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感染泡桐丛枝病类菌原体(PWB-MLO)的泡桐组培苗表现出典型的丛枝症状。电子显微镜和DAPI荧光显微镜检查结果显示病苗的症状严重度与其韧皮部筛管内MLO密度成正相关。HPLC分析病与健株内源激素和酚类物质含量,发现病株游离IAA含量明显低于健康对照,并且IAA含量变化与邻苯二酚含量变化呈显著正相关性(r=0.90,n=11)。细胞分裂素(Z+ZR,iPA)、GA_3和ABA含量变化与MLO侵染关系不密切。病健株抽提液过氧化物酶、IAA氧化酶、多酚氧化酶和苯丙氨酸裂解酶(PAL)活性的差异,以及外源NAA和邻苯二酚处理对病苗与健苗生长的不同影响进一步反映了MLO、邻苯二酚和IAA相互联系和作用的本质。
Paulownia tissue culture inoculated with Paulownia witches’ broom pathogens (PWB-MLO) showed typical symptoms of twig. Electron microscopy and DAPI fluorescence microscopy revealed a positive correlation between the disease severity and the MLO density in the phloem sieve. The contents of endogenous hormones and phenols in diseased and healthy plants were analyzed by HPLC. The results showed that the content of free IAA in diseased plants was significantly lower than that in healthy controls, and there was a significant positive correlation between IAA content and catechol content (r = 0.90, n = 11). The changes of cytokinin (Z + ZR, iPA), GA 3 and ABA contents are not closely related to MLO infection. The differences of the activities of peroxidase, IAA oxidase, polyphenol oxidase and PAL between the extracts of diseased and healthy plants, and the growth of the diseased and healthy seedlings by exogenous NAA and catechol The different influences further reflect the interlinked and functional nature of MLO, catechol and IAA.