论文部分内容阅读
目的 研究口服1,6二磷酸果糖(1,6-Fructose-Diphosphate,FDP)对大剂量异丙肾上腺素所致大鼠脑组织损伤的保护作用,并探讨其保护作用机制。方法 将Wistar大鼠随机分为3组,每组8只(1)ISO组给大鼠皮下注射异丙肾上腺素[isoproterenol,ISO,5mg/(kg·d)],连续7d;(2)FDP组除注射异丙肾上腺素外,同时给予FDP灌胃[10ml/(kg·d)],连续21d;(3)对照组给予等容积生理盐水皮下注射及灌胃。测定脑组织匀浆丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性和总抗氧化能力(T-AOC),用光镜、电镜观察脑组织形态学改变。结果 ISO组、FDP组MDA含量均高于对照组,SOD活性均低于对照组,且脑组织有损害,海马神经元密度均较对照组降低,均P<0.01;但FDP组与ISO组比较,MDA含量低于ISO组,SOD活性和T-AOC均高于ISO组,均P<0.01;FDP组T-AOC与对照组接近,P>0.05,对脑组织的病理损害也较ISO组明显减轻。结论 FDP可预防、治疗慢性心肌缺血坏死所致的缺血性脑组织损伤。FDP的神经保护机制可能与其抗氧化应激、抗自由基、增加细胞内能量、膜稳定等作用有关。
Objective To study the protective effect of oral administration of 1,6-Fructose-Diphosphate (1,6-Fructose-Diphosphate, FDP) on the brain injury induced by high-dose isoproterenol in rats and to explore its protective mechanism. Methods Wistar rats were randomly divided into 3 groups, 8 rats in each group. (1) ISO group was injected subcutaneously with isoproterenol (ISO, 5 mg / (kg · d)] for 7 days. (2) In addition to the injection of isoproterenol, the group was given intragastric FDP [10ml / (kg · d)] for 21 days continuously. (3) The control group was injected subcutaneously with equal volume of normal saline and intragastrically. The contents of malondialdehyde (MDA), superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) in brain homogenate were measured. The morphological changes of brain tissue were observed by light microscope and electron microscope. Results The contents of MDA in ISO group and FDP group were higher than those in control group, and the activity of SOD was lower than that in control group. The damage of brain tissue and neuron densities in hippocampus were lower than those in control group (P <0.01) , MDA content was lower than ISO group, SOD activity and T-AOC were higher than ISO group, P <0.01; TDP in FDP group was close to control group, P> 0.05; Reduce. Conclusion FDP can prevent and treat ischemic brain injury induced by chronic myocardial ischemia and necrosis. The neuroprotective mechanism of FDP may be related to its role of anti-oxidant stress, anti-free radicals, increasing intracellular energy, membrane stability and so on.