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ＨＣＶ相关ＨＣＣ癌组织能检出ＨＣＶＲＮＡ负链，但许多学者检测不到或对其检测方法的准确性产生怀疑。作者收集１３例ＨＣＶ相关ＨＣＣ标本，采用严格的负链检测方法和ＰＣＲ半定量方法系统地比较了ＨＣＣ癌组织和癌旁组织中ＨＣＶ总ＲＮＡ和负链的检出情况及其数量关系。ＨＣＣ癌组织和癌旁组织中，ＨＣＶＲＮＡ负链检出率分别为３０．８％（４／１３）和６９．２％（９／１３），平均滴度分别为３．５和２９．２，两者平均相差８．４倍（０－１００倍）。癌组织中ＨＣＶＲＮＡ负链只有癌旁组织中ＨＣＶ总ＲＮＡ的１／９１．６。本研究结果提示，ＨＣＣ癌组织中的确能检出ＨＣＶＲＮＡ负链，但水平较低。这种可能存在的低水平ＨＣＶ复制对ＨＣＣ的影响可能不大。因而，临床观察和流行病学研究所揭示的ＨＣＶ与ＨＣＣ相关的基础可能存在于ＨＣＣ发生过程的早期，即启动或早期克隆扩增阶段。
HCV-associated HCC cancer tissues can detect HCV RNA negative strands, but many scholars have not detected or doubted the accuracy of their detection methods. The authors collected 13 HCV-associated HCC specimens and systematically compared the detection and quantitative relationship of HCV total RNA and negative strands in HCC cancer tissues and paracancerous tissues using a strict negative-sense detection method and semi-quantitative PCR. The detection rate of negative HCV RNA in HCC and adjacent tissues was 30.8% (4/13) and 69.2% (9/13), respectively. The average titer was 3.5 and 29.2, respectively. The average difference between the two is 8.4 times (0-100 times). The negative HCV RNA in cancer tissue is only 1/91.6 of the total HCV RNA in the paracancerous tissue. The results of this study suggest that HCV RNA negative strands can indeed be detected in HCC cancer tissues, but at a low level. This possible low level of HCV replication may have little effect on HCC. Thus, the clinical observation and epidemiological studies revealed that the basis of HCV-related HCC may exist in the early stages of the HCC process, ie, the initiation or early clonal expansion stage.