论文部分内容阅读
白细胞介素6(IL-6)是一种具有多种效应的细胞因子,能诱导IL-2生成和IL-2R的表达。而且是细胞毒性T细胞分化因子。移植肾急性排斥反应期间血清和尿液IL-6水平增加,提示它可能是急性排斥反应的致病因子。Raasveld等在移植肾急性排斥反应期间对活检物进行免疫组化染色,观察IL-6,了解其局部生成的意义。病人和方法:7例病人均接受基础免疫抑制药物治疗,包括强的松和环孢素。发生急性细胞性排斥反应时,在抗排斥治疗前行肾活检,活检物经过液氮冰冻、做成厚度5μm的恒低温切片,再经过羊抗人重组IL-6多克隆抗体、鼠抗人小管细胞单克隆抗体及抗内皮细胞单克隆抗体等处理,用3-氨基-9-乙基咔
Interleukin-6 (IL-6) is a cytokine that has multiple effects that induce IL-2 production and IL-2R expression. It is also a cytotoxic T-cell differentiation factor. Increased levels of IL-6 in serum and urine during acute rejection of renal allografts suggest that it may be a causative agent of acute rejection. Raasveld et al performed immunohistochemical staining on biopsy specimens during the acute rejection of renal allograft and observed the significance of IL-6 production. Patients and Methods: All 7 patients underwent basic immunosuppressive drug therapy, including prednisone and cyclosporine. In the event of acute cellular rejection, renal biopsy and anti-rejection therapy were performed before biopsy. The biopsy sample was frozen in liquid nitrogen and made into cryostat sections of 5μm in thickness. After passing through a goat anti-human recombinant IL-6 polyclonal antibody, Cell monoclonal antibody and anti-endothelial cell monoclonal antibody treatment, with 3-amino-9-ethyl carbazole