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抗疟药常用测定方法一般是在实验动物体内进行,此法费时长,消耗也大,更值得注意的是动物疟原虫对某些药物的反应与人疟原虫不尽相同。虽然近年来国外已建立起人疟猴模型,但目前在我国用此模型进行试验尚无条件。Trager等体外培养人恶性疟原虫成功后,为体外筛选抗疟药提供了可能性。由于体外培养时可变因素多,影响此方法的准确性与重复性。为此本实验试以半数效量法测算抑制疟原虫繁殖的用药量,以测定药物的抗疟效力。经多次重复试验误差范围不大,为体外测定抗疟药提供了一种较方便的方法。
Antimalarial commonly used assay methods are generally carried out in laboratory animals, this method takes a long time, consumption is also large, more notably animal parasite response to certain drugs and human Plasmodium vary. Although in recent years abroad has established a human malaria model, but currently in our country with this model for testing unconditional. Trager and other in vitro culture of human Plasmodium falciparum after the success of screening for anti-malaria drugs in vitro possibilities. Due to the variable factors in vitro culture, affect the accuracy and repeatability of this method. To this end, this experiment to test the half effective dose inhibition of malaria parasite drug use to determine the efficacy of antimalarial drugs. After repeated trials, the error range is not large, which provides a more convenient method for in vitro antimalarial determination.