论文部分内容阅读
目的探讨间充质干细胞(MSC)诱导骨髓来源的CD11b+Gr1+细胞免疫抑制功能对肿瘤的影响。方法将骨髓中CD11b+Gr1+细胞与MSC共培养后,通过流式细胞术测定CD11b+Gr1+细胞免疫表型,通过T细胞抑制性实验分析T细胞活化情况,采用小鼠4T1乳腺癌模型观察MSC通过CD11b+Gr1+细胞对肿瘤生长的影响。结果 MSC能够促进骨髓中CD11b+Gr1+细胞的存活和生成,同时可诱导正常小鼠骨髓中CD11b+Gr1+细胞对T细胞的抑制作用。动物实验结果显示,MSC处理后的骨髓CD11b+Gr1+细胞可以促进肿瘤的生长,加速小鼠的死亡。结论 MSC可以通过促进骨髓来源的CD11b+Gr1+细胞的抑制作用加速肿瘤的生长进程。
Objective To investigate the effects of mesenchymal stem cells (MSCs) on tumor immunosuppression induced by bone marrow-derived CD11b + Gr1 + cells. Methods CD11b + Gr1 + cells were co-cultured with MSCs in bone marrow. The immunophenotypes of CD11b + Gr1 + cells were determined by flow cytometry. T cell activation was analyzed by T cell suppressive assay. MSCs were observed by 4T1 mouse breast cancer model Effect of CD11b + Gr1 + Cells on Tumor Growth. Results MSC could promote the survival and production of CD11b + Gr1 + cells in bone marrow and induce the inhibition of T cells by CD11b + Gr1 + cells in normal mouse bone marrow. Animal experiments show that the bone marrow CD11b + Gr1 + cells treated with MSC can promote tumor growth and accelerate the death of mice. Conclusion MSC can accelerate tumor growth by promoting the inhibition of bone marrow-derived CD11b + Gr1 + cells.