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目的观察健脾消癌方对结直肠癌裸鼠转移模型组织中血管内皮生长因子(VEGF)及血管内皮抑素(ES)表达的影响,探讨健脾消癌方拮抗结直肠癌转移的作用机制。方法将BALB/c-nu裸鼠按随机数字表随机抽取8只作为对照组,其余裸鼠采用盲肠内注射50μL人结肠癌HCT116细胞造模,3 d后取存活裸鼠32只,随机分为健脾消癌方低、高剂量(10、40 g/kg)组,5-氟尿嘧啶(5-FU,0.13 g/kg)组,模型组,分别ig或ip相应药物及生理盐水4周后,采用q RT-PCR法测定裸鼠结直肠癌转移后结肠、肝、肺及盲肠肿瘤组织中VGEF、ES m RNA表达量。结果在VEGF m RNA表达量方面,各组均较对照组升高,模型组与5-FU组最高(P<0.01);健脾消癌方2组较模型组显著降低(P<0.05、0.01)。在ES m RNA表达量方面,各组均较对照组降低,模型组与5-FU组最低(P<0.01);健脾消癌方2组较模型组显著升高(P<0.05、0.01)。4个部位以盲肠组织VEGF水平最低,ES水平基本相当。结论健脾消癌方可能通过使结肠、肝、肺等组织VEGF的降低与ES的升高来达到拮抗结直肠癌转移的目的。
Objective To observe the effect of Jianpi XiaoJian Fang on the expression of vascular endothelial growth factor (VEGF) and endostatin (ES) in the metastasis model of colorectal cancer in nude mice and to explore the mechanism of action of Jianpi Xiaoyao Fang in antagonizing the metastasis of colorectal cancer . Methods BALB / c-nu nude mice were randomly selected according to a random number table as the control group. The remaining nude mice were injected intracerebroventricularly with 50 μL of human colon cancer HCT116 cells and 32 survived nude mice were randomly divided into Spleen and Kidney Decoction of low, high dose (10,40 g / kg) group, 5-fluorouracil (5-FU, 0.13 g / kg) group, model group, respectively ig or ip corresponding drugs and saline for 4 weeks, The expression of VGEF and ES m RNA in colon, liver, lung and cecum tumor tissues after the metastasis of colorectal cancer in nude mice was detected by q RT-PCR. Results The expression of VEGF m RNA in each group was higher than that in the control group, the highest in the model group and the 5-FU group (P <0.01), and the other two groups were significantly lower than the model group (P <0.05, 0.01) ). Compared with the control group, the expression of ES mRNA in each group was lower than that in the control group (P <0.01), and that in Jianpi Xiaohua Decoction group was significantly higher than that in the model group (P <0.05, 0.01) . In the four sites, the lowest level of VEGF was found in the cecum, and the ES level was basically the same. Conclusion Jianpi Xiaowanfang may achieve the purpose of antagonizing the metastasis of colorectal cancer by reducing the VEGF and increasing the ES in the colon, liver and lung tissues.