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目的探讨应用聚合酶链反应技术（PCR）早期快速诊断单疱病毒性角膜炎的可行性。方法应用PVR测定各种类型的角膜炎病人97例，其中单疱病毒性角膜炎72例，细菌性角膜溃疡15例，真菌性角膜溃疡10例。分别采集各病人角膜病变处分泌物，按PCR操作常规进行检测。结果收集各种类型的角膜炎病人97例，其中男性59例，女性38例，平均42．6岁。行PCR检测后，阳性65例，阳性率为67．0％，其中单疱病毒性角膜炎72例．阳性65例，阳性率为90．3％。细菌性角膜溃疡15例，真菌性角膜溃疡10例，均未测到阳性结果、结论应用PCR在诊断单疱病毒性角膜炎方面具有敏感性高，特异性强，操作简便快捷，可用于单疱病毒性角膜炎的早期快速诊断。
Objective To investigate the feasibility of early diagnosis of herpes simplex virus keratitis by polymerase chain reaction (PCR). Methods 97 cases of various types of keratitis were examined by PVR, including 72 herpes simplex keratitis, 15 bacterial corneal ulcer and 10 fungal corneal ulcer. The patients were collected corneal lesions secretions, according to PCR routine testing. Results A total of 97 patients with various types of keratitis were collected, including 59 males and 38 females, with an average of 42.6 years. After PCR test, 65 cases were positive, the positive rate was 67.0%, including 72 cases of herpes simplex virus keratitis. Positive 65 cases, the positive rate was 90.3%. Bacterial corneal ulcer in 15 cases, fungal corneal ulcer in 10 cases, no positive results were found, the application of PCR in the diagnosis of herpes simplex virus keratitis has high sensitivity, specificity, easy operation, can be used for single blisters Early rapid diagnosis of viral keratitis.