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本研究的目的是为了进一步证实感染日本血吸虫病的家兔血清中的自身抗体。采用间接荧光技术。在家兔感染日本血吸虫病后32～39周内,收集其血清,使之与4微米厚的正常同种异体兔肝冰冻切片起反应,此为第1步;第2步用特异的羊抗兔免疫球蛋白G。荧光度自-至+++分为5级,其中±系指1个视野中少数肝细胞的细胞膜上有微弱荧光着染。在发现感染动物的血清对肝细胞抗原决定簇显示出某种亲和力以后,从10只动物收集一系列血清,使与同种异体肝起反应,以测出抗肝抗体的最高滴度。
The purpose of this study was to further confirm the autoantibodies in sera from rabbits infected with Schistosoma japonicum. Indirect fluorescence technology. After 32 to 39 weeks of infection of Schistosoma japonicum in rabbits, the serum is collected and reacted with 4-micrometer-thick normal rabbit liver frozen sections, which is the first step. Step 2 is performed with specific goat anti- Rabbit immunoglobulin G. Fluorescence from - to +++ is divided into 5 levels, where ± means weak fluorescence staining on the cell membrane of a few hepatocytes in one field of view. After the sera from infected animals were found to show some affinity for the hepatocyte epitopes, a series of sera from 10 animals were collected and reacted with allogeneic liver to determine the highest titer of anti-liver antibody.