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以芦笋两性株自交S1代雄株为试验材料,首先与优良雌株进行测交,然后利用与芦笋性别决定基因紧密连锁的显性DNA分子标记对苗期各单株进行性别鉴定,推断出超雄株,为芦笋全雄育种提供亲本材料。研究结果表明:显性标记 Asp1-T7 和 Asp1-T7sp特异性好,扩增效果稳定,均能够鉴别芦笋雌、雄性别;一共测验了14 株芦笋两性株自交 S1代的雄株,从中筛选出2株超雄株;对田间测交组合单株显花后性别表型调查的结果表明,DNA 分子标记检测结果与田间实际相符,2株超雄株所对应的组合被确证为全雄组合。
Asparachis male parent selfing male S1 was used as test material, and the female parent plants were firstly crossed with elite female parent plants. Then the sex determination of each individual in seedling stage was carried out using the dominant DNA marker closely linked to sex determination genes of asparagus Super male plant, providing all parents of asparagus breeding breeding materials. The results showed that the dominant markers Asp1-T7 and Asp1-T7sp had good specificity and stable amplification effect, and were able to identify the male and female asparagus. A total of 14 male asparagus selfing male S1 plants were tested and screened The results showed that the results of DNA molecular markers were in line with the actual situation in the field. The combination of two supernova plants was confirmed as the combination of all male .