在pH 7.0的B-R缓冲溶液中,基于抗坏血酸对色氨酸、酪氨酸、苯丙氨酸等芳香族氨基酸荧光的猝灭作用,建立了荧光猝灭法测定抗坏血酸的新方法。在最适宜条件下,方法的线性范围和检出限分别为:0.1—20mg/L、0.045mg/L(色氨酸体系);0.05—15mg/L、0.032mg/L(酪氨酸体系);0.01—5mg/L、0.0038mg/L(苯丙氨酸体系)。方法已应用于实际样品的测定并对反应机理进行了探讨。 A new fluorescence quenching method for the determination of ascorbic acid was established based on the quenching effect of ascorbic acid on the fluorescence of aromatic amino acids such as tryptophan, tyrosine and phenylalanine in pH 7.0 B-R buffer solution. Under the most suitable conditions, the linear range and the detection limit of the method were 0.1-20mg / L, 0.045mg / L (tryptophan system), 0.05-15mg / L, 0.032mg / L (tyrosine system) ; 0.01-5mg / L, 0.0038mg / L (Phenylalanine system). The method has been applied to the determination of the actual samples and the reaction mechanism was discussed.