The work is to explore the relationship between the levels of cytokines(IL-1β and IL-6)in C57BL/6J micetreated with MPTP and brain lateralization.By using paw preference test,right-pawed,left-pawed micemodels were established.Following single injection of 1-methyl-4-phenyl-2,3,6-tetrahydropyrid(MPTP)(40mg/kg)to impair dopaminergic neuron,enzyme linked immunosorbent assay(ELISA)kits were used fordetection of plasma levels of cytokines.The results showed that in saline treated C57BL/6J mice(control),therewas no obvious difference observed between left-pawed and right-pawed mice in plasma levels of IL-1β andIL-6.In MPTP treated mice,there was no difference between level of IL-1β in left-pawed mice and that inright-pawed ones in statistics,that is,they were increased on day 1 and day 3,but decreased on day 6.Theplasma level of IL-6 was lower in left-pawed than that in right-pawed mice (p<0.005)after MPTP treatment.On day 1 and day 3,the level of IL-6 was almost the same as control;on day 6,it was significantly increased,higher than that of control(p<0.001)in left-pawed mice.While in right-pawed mice,on day 1 and day 3,it wasno different from control,too.And on day 6,it significantly increased in compared with control(p<0.005).Inconclusion,the level of plasma IL-6 of C57BL/6J mice treated with MPTP increased.The variation of IL-6 wascorrelated to brain lateralization.Cellular & Molecular Immunology.2004;1(3):219-223. The work is to explore the relationship between the levels of cytokines (IL-1β and IL-6) in C57BL / 6J micetreated with MPTP and brain lateralization. By using paw preference test, right-pawed, left-pawed mice models were established. Popular single injection of 1-methyl-4-phenyl-2,3,6-tetrahydropyrid (MPTP) (40 mg / kg) to impair dopaminergic neuron, enzyme linked immunosorbent assay (ELISA) kits were used for removal of plasma levels of cytokines. showed that in saline treated C57BL / 6J mice (control), therewas no obvious difference observed between left-pawed and right-pawed mice in plasma levels of IL-1β and IL-6. MPMP treated mice, there was no difference between level of IL-1β in left-pawed mice and that inright-pawed ones in statistics, that is, they were increased on day 1 and day 3, but decreased on day 6. Theplasma level of IL-6 was lower in left-pawed than that that in right-pawed mice (p <0.005) after MPTP treatment. On day 1 and day 3, the level of IL-6 was almost the same as control; on da it was significantly increased, higher than that of control (p <0.001) in left-pawed mice. Shift in right-pawed mice, on day 1 and day 3, it wasno different from control, too. On on day 6 , it was significantly increased in compared with control (p <0.005). Inconlusion, the level of plasma IL-6 of C57BL / 6J mice treated with MPTP increased. The variation of IL-6 wascorrelated to brain lateralization. Cellular & Molecular Immunology. 2004 ; 1 (3): 219-223.