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用重复序列探针MGR586与限制性内切酶EcoR1组合,分析了在我国1980~1996年期间的17省(市)146个不同稻区475个稻瘟病菌株(下简称菌株)的限制性片段长度多态性(RFLPs),依其MGR-DNA指纹(下简称指纹)的相似率,结合病菌的致病性测定,将表现为48个不同致病型的475个菌株区分为56个谱系,每个谱系的寄主范围有限,且与不同稻区稻瘟病的群体结构差异明显.菌株的指纹分析和致病性测定结果表明,稻瘟菌有远距离传播的可能性,并在适宜的气候和人工接种条件下,草瘟菌和稻瘟菌可彼此互交.文中,作者还分析了不同稻区稻瘟菌谱系和毒性之间的关系,以期明确该菌的致病性变异及其演化过程,为改进种质资源的筛选方法和持抗育种的程序提供科学依据.
By using the combination of MGR586 and restriction endonuclease EcoR1, the restriction fragment length of 475 M. grisea strains (hereinafter referred to as strains) in 146 different rice regions in 17 provinces (cities) from 1980 to 1996 was analyzed. According to the similarity of MGR-DNA fingerprinting (fingerprinting) and the pathogenicity of pathogenic bacteria, 475 strains of 48 different pathogenicity were divided into 56 lines The host range of the pedigree is limited, and there is significant difference with the population structure of rice blast in different rice regions. The results of fingerprinting and pathogenicity test showed that there was a possibility that Magnaporthe grisea could spread long-rangely and that D. grisea and M. grisea could cross each other under suitable climate and artificial inoculation. In this paper, the author also analyzed the relationship between the lineages and toxicity of Magnaporthe grisea in different rice regions in order to clarify the pathogenic mutation of the bacteria and its evolution process, provide scientific methods for screening methods and breeding programs to improve germplasm resources in accordance with.