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目的探讨血栓前状态所致复发性流产(RSA)患者外周血淋巴细胞亚群的变化及其意义。方法收集73例血栓前状态RSA患者(PTS-RSA)外周血,另设22例正常早孕妇女作为正常对照组(HC),采用流式细胞术对其进行淋巴细胞亚群(T细胞、B细胞、NK细胞相关指标)检测,并比较PTS-RSA患者治疗前后相应指标的变化。结果与HC组比较,PTS-RSA组CIK细胞绝对值、NK细胞百分比和CIK细胞百分比明显降低,差异有统计学意义(P<0.05)。PTS-RSA患者在经过临床相关治疗后,NK细胞百分比及绝对值明显下降,差异均有统计学意义(P<0.05);B细胞百分比、Ts细胞百分比明显上升,差异均有统计学意义(P<0.05)。结论血栓前状态可能激活RSA患者体内的一系列免疫反应,对PTS所致RSA患者外周血淋巴细胞亚群进行监测,将对诊断及治疗具有一定的指导意义。
Objective To investigate the changes and significance of peripheral blood lymphocyte subsets in patients with recurrent spontaneous abortion (RSA) caused by prethrombosis. Methods Totally 73 peripheral blood samples from prethrombotic state of RSA patients (PTS-RSA) were collected and 22 normal pregnant women were recruited as normal control group (HC). Flow cytometry was used to detect the percentage of lymphocyte subsets (T cells, B cells , NK cells related indicators) detection, and compared PTS-RSA patients before and after treatment changes in the corresponding indicators. Results Compared with HC group, the absolute value of CIK cells, the percentage of NK cells and the percentage of CIK cells in PTS-RSA group were significantly decreased (P <0.05). The percentage of NK cells and the absolute value of PTS-RSA in patients with PTS-RSA were significantly decreased (P <0.05). The percentage of B cells and the percentage of Ts cells were significantly increased (P <0.05). Conclusion Pre-prothrombotic state may activate a series of immune responses in RSA patients. Monitoring peripheral blood lymphocyte subsets in PTS-induced RSA patients will be of guiding significance in diagnosis and treatment.