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目的:探讨分析不同频率电针治疗时肌腱血流的变化及其变化机制。方法:将40只Wistar大鼠分为2Hz组、10Hz组、100Hz组和针刺(10Hz)+阿托品组、针刺(10Hz)+酚妥拉明组。手术剥离出大鼠胫骨粗隆上缘的髌腱,利用超声测定技术,观察针刺足三里和梁丘穴前后髌腱血流量的变化。并采用静脉滴入阿托品和酚妥拉明的方法,对针刺作用机制进行分析。结果:(1)2Hz组和10Hz组在针刺过程中,髌骨腱血流量分别下降至针刺前的82.11±10.8%、49.17±11.63%,针刺停止后,两组血流量却逆转为高于针刺前的水平,以10Hz组上升幅度较为明显;(2)100Hz组针刺过程中,血流量下降至针刺前的37.54±12.79%,针刺停止后血流量未恢复到针刺前的水平;(3)针刺(10Hz)+阿托品组:针刺时血流量降低至44.61±12.01%,停针后血流量未出现增加现象;(4)针刺(10Hz)+酚妥拉明组:针刺时血流量增加至119.73±14.66%,针刺停止后血流量继续增加。结论:(1)电针刺激期间髌腱血流下降,刺激停止后血流量明显增加;(2)中、低频率刺激对血流量增加效果优于高频刺激;(3)针刺对血流量的影响与舒血管神经和缩血管神经的调节有关。
Objective: To investigate the changes of tendon blood flow and its mechanism of electro-acupuncture at different frequencies. Methods: 40 Wistar rats were divided into 2Hz group, 10Hz group, 100Hz group and acupuncture (10Hz) + atropine group, acupuncture (10Hz) + phentolamine group. The patellar tendon in the superior margin of the tibial tuberosity was dissected out by surgery. The changes of patellar tendon blood flow before and after acupuncture at Zusanli and Liangqiu points were observed by using ultrasonic technique. Atropine and phentolamine were administered intravenously, and the mechanism of acupuncture was analyzed. Results: (1) Patellar tendon blood flow decreased to 82.11 ± 10.8% and 49.17 ± 11.63% respectively in 2Hz and 10Hz groups after acupuncture. The blood flow in both groups reversed after acupuncture stopped (2) In the 100Hz group, the blood flow decreased to 37.54 ± 12.79% before acupuncture, and the blood flow did not recover to the level before acupuncture ; (3) acupuncture (10Hz) + atropine group: blood flow reduced to 44.61 ± 12.01% at acupuncture, blood flow did not appear to increase after acupuncture; (4) acupuncture (10Hz) + phentolamine Group: blood flow increased to 119.73 ± 14.66% at acupuncture, blood flow continued to increase after acupuncture stopped. Conclusions: (1) The blood flow of patellar tendon decreased during the electroacupuncture stimulation, and the blood flow increased significantly after the stimulation was stopped. (2) The effect of medium and low frequency stimulation on blood flow was better than high frequency stimulation; (3) The effects are related to the regulation of vasodilator nerves and vasoconstrictor nerves.