半个多世纪以来,随着方法学和科研技术的发展,人们对雄性生物体内雌激素的认识逐步深入。开始用的生物测定法较灵敏,但不可靠,受多种因素的影响。继之使用物理化学方法,如光谱测定法,荧光测定法和双同位素稀释等,主要用于测定尿中雌激素的含量,不能用于血液或组织样品的测定。其原因是不太灵敏。一直到70年代放射免疫测定法的问世,才能准确和常规地测定血浆和组织样品中的雌激素水平。人们对雌激素的研究才取得了较大的进展。雌激素主要 More than half a century, with the development of methodology and scientific research, people gradually deepen their understanding of estrogen in male organism. The initial bioassay is more sensitive but not reliable and is influenced by many factors. Followed by the use of physical and chemical methods, such as spectrometry, fluorometry and double isotope dilution, mainly for the determination of urinary estrogen content, can not be used for blood or tissue samples. The reason is less sensitive. Until the advent of radioimmunoassay in the 1970s, the level of estrogen in plasma and tissue samples was accurately and routinely determined. People have made great progress in the study of estrogen. Estrogen mainly