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脱水素(dehydrin)在植物抵御非生物逆境胁迫方面具有重要作用。本研究以楸子(Malus prunifolia)为材料,采用改良CTAB法提取RNA,根据苹果的脱水素基因序列设计特异引物,利用反转录聚合酶链式反应(RT-PCR)技术扩增,获得一条楸子脱水素cDNA全长序列。该基因全长874 bp,序列中含有一个720 bp的开放阅读框,编码233个氨基酸,为YK3型脱水素,命名为MpDHN8。氨基酸同源性分析表明,该基因编码的氨基酸序列与苹果(Malus domestica)脱水素基因(AFG33216.1)的同源性高达97%。荧光定量PCR结果表明,在干旱和盐胁迫下,MpDHN8的表达量显著提高,说明该基因作为正向调控因子参与调控楸子的逆境胁迫。
Dehydrins play an important role in plants against abiotic stresses. In this study, we use the improved CTAB method to extract the RNA from Malus prunifolia, design specific primers according to the dehydrin gene sequence of apple and amplify it by reverse transcription-polymerase chain reaction (RT-PCR) to obtain one Catalpa seed dehydrin full-length cDNA sequence. The full length of this gene is 874 bp, which contains a 720 bp open reading frame and encodes 233 amino acids. It is named as YK3 dehydratase and named as MpDHN8. Amino acid homology analysis showed that the deduced amino acid sequence of this gene shared 97% homology with the Malus domestica dehydratoxin gene (AFG33216.1). Fluorescence quantitative PCR results showed that under drought and salt stress, the expression of MpDHN8 was significantly increased, indicating that the gene as a positive regulator involved in regulating Catalpa bungei stress stress.