以陇东地区野生紫花苜蓿无菌苗的下胚轴、子叶、叶片和叶柄为外植体,MS为基本培养基,研究划破种皮以及不同生长调节物质种类与配比对愈伤组织诱导和分化影响的结果表明:划破种皮可提高种子发芽率;在外植体中下胚轴的愈伤组织诱导率最高,达92.2%;最佳愈伤组织诱导培养基为MS+2,4-D2.0mg·L-1(单位下同)+NAA1.0+2.5%蔗糖+0.6%琼脂,最佳分化诱导培养基为MS+KT0.5+NAA0.3+2.5%蔗糖+0.6%琼脂,成苗培养基为1/2MS+NAA0.1+1%蔗糖+0.6%琼脂。 Hypocotyls, cotyledons, leaves and petiole of wild alfalfa seedlings in Longdong were used as explants. MS was used as the basic medium to study the effects of the seed coat and different growth-regulating substances on the callus induction The results showed that the seed germination rate could be improved by scratching the seed coat, the callus induction rate of hypocotyls reached the highest (92.2%) in the explants, and the best callus induction medium was MS + 2,4 -D2.0mg · L-1 (the same below) + NAA1.0 + 2.5% sucrose + 0.6% agar, the best differentiation induction medium was MS + KT0.5 + NAA0.3 + 2.5% sucrose + 0.6% agar , The medium for seedlings was 1 / 2MS + NAA0.1 + 1% sucrose + 0.6% agar.