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目的探讨重组人促红细胞生成素(recombinant human erythropoietin,rhEPO)干预对缺氧复氧后HK-2细胞内视神经萎缩症蛋白(optic atrophy 1,OPA1)表达变化及细胞凋亡率的影响。方法体外培养HK-2细胞随机分为正常对照组、H/R组和rhEPO干预组。然后免疫组化检测各组细胞胞质内OPA1蛋白表达变化,流式细胞仪检测各组细胞凋亡数量差异。结果与正常对照组比较,H/R组细胞数量减少,细胞凋亡率显著增加,同时细胞内OPA1表达减少;而与H/R组相比,rhEPO干预组细胞数量增加,细胞凋亡率下降,OPA1表达明显增强,但各指标仍未恢复至正常对照组水平。结论rhEPO对缺氧-复氧所致肾小管上皮细胞损伤具有保护作用,其机制可能通过抑制细胞内OPA1蛋白表达而实现。
Objective To investigate the effect of recombinant human erythropoietin (rhEPO) on the expression of OPA1 and the apoptotic rate of HK-2 cells after hypoxia-reoxygenation. Methods HK-2 cells cultured in vitro were randomly divided into normal control group, H / R group and rhEPO intervention group. Then the expression of OPA1 protein in the cytoplasm of each group was detected by immunohistochemistry, and the difference of the number of apoptotic cells in each group was detected by flow cytometry. Results Compared with normal control group, the number of cells in H / R group decreased and the apoptosis rate increased significantly, while the expression of OPA1 decreased in H / R group. Compared with H / R group, the number of cells in rhEPO group decreased and the apoptosis rate decreased , OPA1 expression was significantly enhanced, but the indicators have not returned to the level of the normal control group. Conclusion rhEPO can protect renal tubular epithelial cells induced by hypoxia-reoxygenation and its mechanism may be through inhibiting the expression of OPA1 protein.