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将以黄芪为主药并加入另外5味中草药所配伍的复方中草药粉碎制剂,以质量分数分别为1%、2%、3%和4%的剂量添加于基础饲料中,连续投喂刺参24 d,以基础饲料饲喂组为对照。于投喂后第7天、第10天、第14天、第18天和第24天测酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、一氧化氮合酶(NOS)与超氧化物歧化酶(SOD)活性。结果表明:投喂后24 d内,各试验组ACP、NOS与SOD活性均随时间延长先升高后降低,AKP活性随时间延长而升高。2%复方中草药组的ACP活性在投喂后第14天活性最高,值为2.91 U/100 mL;SOD活性在投喂后第18天最高,值为137.08 U/mL;投喂后第24天时AKP活性最高,为3.05金氏单位/100 mL。与对照组具有显著性差异;1%复方中草药组的NOS活性在投喂后第18天最高,值为10.76 U/mL,与对照组具有显著性差异。研究结果表明:饲料中添加复方中草药能提高刺参免疫,建议其适宜添加量为2%复方中草药。
Astragalus will be the main drug and add another 5 herbs Chinese herbal medicine compatibility of the compound Chinese herbal medicine crushing agent, the mass fraction of 1%, 2%, 3% and 4% of the dose added to the basic feed, continuous feeding of sea cucumber 24 d, fed with basal diet as control. The activities of acid phosphatase (ACP), alkaline phosphatase (AKP), nitric oxide synthase (NOS) and superoxide dismutase (SOD) were measured on the 7th day, the 10th day, the 14th day, the 18th day and the 24th day after the feeding. Dismutase (SOD) activity. The results showed that the activities of ACP, NOS and SOD in each experimental group increased first and then decreased with time, and AKP activity increased with time. The activity of ACP in 2% compound Chinese herbal medicine group was the highest on the 14th day after feeding, with a value of 2.91 U / 100 mL. The SOD activity was highest on the 18th day after the feeding, with a value of 137.08 U / mL. On the 24th day after feeding, The highest AKP activity was 3.05 gold units / 100 mL. Compared with the control group, the NOS activity of 1% compound Chinese herbal medicine group was the highest on the 18th day after feeding, with a value of 10.76 U / mL, which was significantly different from the control group. The results showed that adding compound herbs to the feed could improve the immunity of the sea cucumber, and it is suggested that the suitable dosage should be 2% compound Chinese herbal medicine.