论文部分内容阅读
目的 探讨通过分析食管癌高、低发区人群食管癌前病变细胞核增殖抗原 (PCNA)分子变化特征及其与形态学变化的关系 ,加深对食管癌变分子学机制的了解 ,并为临床肿瘤生物防治提供重要的理论基础和手段。方法 采用组织病理学和免疫组织化学 (ABC)法 ,检测食管癌高发区 15 68例及低发区 3 2 8例无症状人群内镜普查活检标本组织中细胞增生核抗原 (PCNA)分子变化与病变的关系。结果 PCNA的免疫阳性率在食管癌高、低发区活检组织中从正常上皮→基底细胞过度增生 (BCH)→间变 (DYS)呈升高趋势 ;定量分析显示 ,高发区居民食管正常上皮及各级病变组织中 ,单位面积的阳性细胞数均显著高于低发区形态学相似的各级病变组织 (P <0 0 5 )。结论 PCNA的变化能够比较灵敏的反映食管上皮细胞增生状况 ,并可能是高危人群筛查的重要检测指标。高、低发区无症状居民形态学相似的癌前病变其PCNA阳性细胞数明显不同 ,提示二者可能存在不同的细胞增生调控机制 ,并可能存在不同的环境致癌危险因素
Objective To investigate the molecular characteristics of PCNA in esophageal precancerous lesions of esophageal high and low prevalence areas and its relationship with morphological changes and to deepen the understanding of molecular mechanisms of esophageal cancer and to provide evidences for the prevention and treatment of clinical tumor biological control Provide an important theoretical basis and means. Methods The histopathological and immunohistochemical (ABC) methods were used to detect the molecular changes of PCNA in 15 68 cases of esophageal cancer and 382 cases of asymptomatic in low incidence area. The relationship between the lesion. Results The positive rate of PCNA in the high and low incidence of esophageal cancer biopsy tissue from normal epithelium → basal cell hyperplasia (BCH) → interdifferentiation (DYS) showed an upward trend; quantitative analysis showed that high incidence of esophageal normal epithelium and At all levels of lesion, the number of positive cells per unit area was significantly higher than that in the lesion at all levels (P <0 05). Conclusion The changes of PCNA can more sensitively reflect the proliferation of esophageal epithelial cells and may be an important detection index for high-risk population screening. The number of PCNA positive cells in precancerous lesions of asymptomatic residents in high and low incidence areas was significantly different, suggesting that there may exist different mechanisms of cell proliferation regulation and possibly different environmental carcinogenic risk factors