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目的 :探讨肝硬化患者血清透明质酸 (HA)、IL -6、IL -8的浓度变化。方法 :对 12 7例正常人、急慢性肝炎及肝硬化病人 ,采用放免法测定其血清HA ;以ELISA双抗体夹心法测定血清IL -6、IL -8浓度 ,并对HA、IL -6、IL -8结果与α球蛋白及A/G间分别作直线相关分析。结果 :肝硬化组血清HA、IL -6、IL -8结果分别为 (5 5 4.0±2 2 1.7) μg/L、(2 3 2 .8± 10 .1)pg/ml、(197.6± 7.5 )pg/ml ,均较其它组明显升高 ;相关分析结果显示 ,血清三项指标与α球蛋白及A/G比例分别呈正负相关趋势。结论 :HA、IL -6、IL -8在肝硬化的进程中起一定的协同作用 ,三者升高可反应肝硬化的程度 ,提示三者的检测为肝硬化的诊断可提供一定的血清学依据。
Objective: To investigate the changes of serum hyaluronic acid (HA), IL-6 and IL-8 in patients with cirrhosis. Methods: Serum HA was measured by radioimmunoassay in 127 normal subjects, patients with acute and chronic hepatitis and cirrhosis. The concentrations of IL-6 and IL-8 in serum were measured by double antibody sandwich ELISA. The levels of HA, IL-6, IL -8 results and alpha globulin and A / G were analyzed for linear correlation. Results: The serum levels of HA, IL-6 and IL-8 in cirrhosis group were (55.03 ± 22.7) μg / L, (23.2 ± 10.1) pg / ml and ) pg / ml, were significantly higher than other groups; correlation analysis showed that serum three indicators and α-globulin and A / G ratio were positive and negative trends. CONCLUSIONS: HA, IL-6 and IL-8 play a synergistic role in the progression of liver cirrhosis. The increase of the three can reflect the degree of cirrhosis, suggesting that the detection of the three may provide some serological evidence for the diagnosis of cirrhosis in accordance with.