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以地黄根茎所获无菌苗为材料,对其愈伤组织诱导、分化和再生植株的获取进行了初步研究.结果表明:取叶片、茎段、叶柄进行愈伤组织诱导,筛选出最适培养基为MS附加2,4-D0.5mg/L、BA1.0mg/L,愈伤组织诱导率可达100%.将叶片接种在分化培养基中,诱导不定芽,其最适分化培养基为MS附加BA3mg/L、NAA0.1mg/L,分化率为77.5%.试管苗在改良的MS 大量与微量元素、铁盐和有机物质各1/2 附加NAA0.05mg/L的培养基上,经过15～20d培养,生根率可达100%.
Taking the germ-free shoots of Rehmannia glutinosa rhizome as material, the callus induction, differentiation and regeneration of callus were preliminarily studied.The results showed that callus induction from leaves, stem segments and petiole, The basal MS supplemented with 2,4-D0.5mg / L, BA1.0mg / L, callus induction rate of up to 100%. The leaves were inoculated in differentiation medium to induce adventitious buds, the optimum culture medium for MS supplemented with BA3mg / L, NAA0.1mg / L, the rate of differentiation was 77.5% .Treatment of test-tube seedlings in a large number of improved MS and trace elements, iron salts and organic matter each additional 1/2 NAA0.05mg / L medium, 15 ~ 20d culture, rooting rate up to 100%.