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目的观察大蒜素对肠道细胞系Caco-2细胞胆固醇外排及胆固醇转运基因ABCG5/ABCG8表达的影响,探讨大蒜素对肠道细胞胆固醇转运的影响及机制。方法 Caco-2细胞随机分为对照组,胆固醇微胶粒组,胆固醇微胶粒加大蒜素组和大蒜素组4组。处理末,采用油红O染色观察细胞内脂滴,高效液相色谱法测细胞内胆固醇含量,免疫印迹法测ABCG5和ABCG8的蛋白表达。结果与对照组比较,胆固醇微胶粒增加Caco-2细胞内脂滴和胆固醇含量(P<0.05),而大蒜素减少胆固醇微胶粒处理后细胞内脂滴和胆固醇含量,增加细胞内胆固醇排出率(70.30%比20.92%,P<0.05)。大蒜素显著上调Caco-2细胞ABCG5和ABCG8的蛋白水平。结论大蒜素促进细胞内胆固醇排出,其机制可能与上调ABCG5和ABCG8表达相关。
Objective To observe the effects of allicin on cholesterol efflux and expression of cholesterol transport gene ABCG5/ABCG8 in intestinal cell line Caco-2 cells, and to explore the effects of allicin on intestinal cholesterol transport and its mechanism. Methods Caco-2 cells were randomly divided into control group, cholesterol micelle group, cholesterol micelles plus allicin group and allicin group. At the end of treatment, intracellular lipid droplets were observed by oil red O staining. The intracellular cholesterol content was measured by high performance liquid chromatography, and protein expressions of ABCG5 and ABCG8 were measured by Western blotting. Results Compared with the control group, cholesterol micelles increased the lipid droplets and cholesterol content in Caco-2 cells (P<0.05), while allicin reduced lipid droplets and cholesterol levels in cholesterol-treated micelles and increased intracellular cholesterol excretion. The rate (70.30% vs 20.92%, P<0.05). Allicin significantly up-regulated the protein levels of ABCG5 and ABCG8 in Caco-2 cells. Conclusion Allicin promotes cholesterol excretion in cells, and its mechanism may be related to up-regulation of ABCG5 and ABCG8 expression.