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目的 :克隆人白细胞介素 18(IL - 18)全长cDNA基因 ,进一步探讨人白细胞介素 18的功效。方法 :采用RT -PCR的方法从人胚肝组织中扩增出人白细胞介素 18(IL - 18)基因 ,并克隆到真核表达的绿色荧光蛋白报告载体pEGFP -N1中。结果 :经PCR及DNA序列测定得以证实其序列与文献报导一致。结论 :该实验成功地克隆了IL - 18cDNA ,并将其重组构建了含有IL - 18cDNA的真核表达载体pEGFP -N1-IL18。
Objective: To clone the full - length cDNA of human interleukin - 18 (IL - 18) and further explore the efficacy of human interleukin - 18. Methods: Human interleukin 18 (IL - 18) gene was amplified from human embryonic liver tissue by RT - PCR and cloned into eukaryotic expression vector pEGFP - N1. Results: The PCR and DNA sequencing confirmed its sequence consistent with the literature. Conclusion: The experiment successfully cloned IL - 18 cDNA and constructed the eukaryotic expression vector pEGFP - N1 - IL18 containing IL - 18 cDNA.