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骨髓基质细胞是造血微环境的重要组成部分,除对造血细胞有物(?)持作用外,还分泌多种影响造血细胞生长的因。笔者从胎儿骨髓中分离出一株纤维母细胞系,并进行亚克隆。将其条件培养液进行部分纯化,观察纯化的活性物质对小鼠造血细胞增殖分化的影响。 材料与方法 一、HFCL-1条件培养液的制备:HFCL-1细胞来自胎儿骨髓。其克隆亚系HFCL-1细胞在含5％小牛血清的IMDM培养液中滚动培养,每72小时收集条件培养液1次,备生化分离。
Bone marrow stromal cells are an important part of the hematopoietic microenvironment, in addition to the role of the hematopoietic cells (?) Hold, but also secrete a variety of factors that affect hematopoietic cell growth. The author isolated a fibroblast cell line from fetal bone marrow and subcloned. The conditioned medium was partially purified to observe the effect of the purified active substance on proliferation and differentiation of mouse hematopoietic cells. Materials and methods A, HFCL-1 conditioned medium preparation: HFCL-1 cells from fetal bone marrow. The clone subfamily HFCL-1 cells were scrolled in IMDM medium containing 5% fetal bovine serum and the conditioned medium was collected once every 72 hours for biochemical isolation.