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应用２．２．１５细胞（ＨＢＶ基因转染的ＨｅｐＧ２细胞株）转种裸鼠，建立荷瘤裸鼠ＨＢＶ动物模型。受种裸鼠皮下可迅速长出移植瘤并分泌ＨＢＶ多项标志物至外周血液中。应用互补于ＨＢＶｍＲＮＡＳＰⅡ增强子区的１５聚反义硫代寡核苷酸以２０μｇ／ｇ鼠体重剂量连续治疗１０ｄ，结果治疗组荷瘤裸鼠血清中ＨＢｓＡｇ、ＨＢｅＡｇ及ＨＢＶ－ＤＮＡ均受到明显抑制。证明该反义核酸在动物体内同样具有良好的抗病毒效应，为从基因水平研制新一代抗乙肝病毒药物奠定了基础。
2.2.15 cells (HepG2 cell line transfected with HBV gene) were transplanted into nude mice to establish an animal model of HBV in nude mice. Under nude mice subcutaneously can quickly grow xenografts and secrete a number of markers of HBV to peripheral blood. The 15-antisense phosphorothioate oligonucleotides complementary to the HBV mRNASPII enhancer region were continuously treated with 20 μg / g mouse body weight for 10 days. The results showed that the serum HBsAg, HBeAg and HBV-DNA of the tumor-bearing nude mice were significantly inhibited. Proved that the antisense nucleic acid also has a good antiviral effect in animals and laid the foundation for the development of a new generation of anti-hepatitis B virus drugs at the gene level.