论文部分内容阅读
目的建立UPLC-MS快速定量检测葡萄酒中甜蜜素的方法。方法采用超高效液相色谱串联三重四极杆质谱仪,色谱条件:Agela Venusil-C18(2.1 mm×50 mm,1.7μm)色谱柱,柱温40℃,流动相为:乙腈=0.01mol/L,乙酸铵=95∶5,流速为0.25 ml/min。质谱条件:ESI源,选择离子监测(SIM)。以定性离子对之间的相对丰度定性,以定量离子对峰面积定量。结果甜蜜素在0.005 0～0.557 4 mg/L范围内呈现良好的线性关系(r=0.999 8);平均加样回收率均在91.14%～100.82%;甜蜜素含量重复性试验RSD<4.779%。结论本方法定性可靠、定量准确,且快速简便,可用于葡萄酒中甜蜜素的检测。
OBJECTIVE To establish a rapid and quantitative method for UPLC-MS determination of cyclamate in wine. Methods The chromatographic conditions were as follows: Agela Venusil-C18 (2.1 mm × 50 mm, 1.7 μm) column with a mobile phase of acetonitrile = 0.01 mol / L , Ammonium acetate = 95: 5, flow rate 0.25 ml / min. MS Conditions: ESI source, Selected Ion Monitoring (SIM). The relative abundance of qualitative ion pairs was characterized qualitatively to quantitate the peak area. Results Cyclamen showed a good linearity (r = 0.999 8) in the range of 0.005 0-0.557 4 mg / L. The average recoveries were 91.14% -100.82%. The cyclin content repeatability test RSD was 4.779%. Conclusion The method is qualitative and reliable, accurate and accurate, and fast and easy. It can be used for the determination of sodium cyclamate in wine.