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目的 :观察胚胎肝Sca - 1+细胞能否在体外向神经组织细胞分化。方法 :免疫磁珠法分离孕 14 5dC5 7BL/6J小鼠的胚胎肝的Sca - 1+细胞 ,以DMEM/F12 + 10 %胎牛血清培养液培养 ,当细胞融合达 80 % ,按 1∶3比例传代。第 5代细胞用BME和RA先后诱导 2 4h ,再用无血清培养基培养 5h至 5d。免疫细胞化学检测细胞特点。结果 :胚胎肝Sca - 1+细胞经诱导后表现神经元形态 ,并表达神经元特异蛋白如神经元特异性核蛋白 (NeuN)、神经丝蛋白M、神经元特异性微管蛋白 1(TuJ- 1) ,但是无微管相关蛋白Tau、MAP - 2和星形胶质细胞特异酸性蛋白 (GFAP)表达。结论 :胚胎肝Sca- 1+细胞 (主要为造血干细胞 )具有可塑性 ,在体外能分化为早期未成熟的神经元样细胞。这提示胚胎肝细胞可能用于神经系统疾病的细胞治疗和基因治疗
Objective: To observe whether embryonic hepatic Sca - 1 + cells can differentiate into nerve tissue cells in vitro. METHODS: Sca - 1 + cells from embryonic liver of pregnant mice were isolated by immunomagnetic beads method and cultured in DMEM / F12 + 10% fetal bovine serum. When the cells reached 80% confluency, Proportion of the passage. The 5th generation cells were induced with BME and RA for 24 h and then cultured in serum-free medium for 5 h to 5 d. Immunocytochemistry to detect cell characteristics. Results: The embryonic liver Sca - 1 + cells showed neuronal morphology after induction and expressed neuron - specific protein such as NeuN, Neurofilament M, TuJ - 1), but no microtubule - associated protein Tau, MAP - 2 and astrocyte - specific acidic protein (GFAP) expression. Conclusion: The embryonic hepatic Sca-1 cells (mainly hematopoietic stem cells) are plastic and can differentiate into early immature neuron-like cells in vitro. This suggests that embryonic hepatocytes may be used for cell therapy and gene therapy of neurological diseases