兔半月板纤维软骨细胞的体外培养,常用Webber氏法。我们在实际工作中发现该法存在如下问题:(1)用酶消化分离后,细胞成活率低;(2)培养条件高,如需要加入一些细胞生长因子等。为解决这些不足,我们采用“酶消化——组织块培养”联合法分离细胞,发现此法比单纯用酶消化法取得较满意的结果。现介绍如下: Rabbit meniscus fibroblasts cultured in vitro, commonly used Webber’s method. We found in the actual work of the law the following problems: (1) digested with enzymes, cell survival rate is low; (2) high culture conditions, such as the need to add some cell growth factors. To solve these problems, we use “enzyme digestion - tissue culture” method to separate cells, found that this method than the simple enzymatic digestion to obtain more satisfactory results. Now introduced as follows: