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Sensing of analytes in biological fluids and biotechnological production media remains a challenge. Here, the luminescent response of the europium (Ⅲ) complex of 1,4,7,10-tetraazacyclododecane-1,4,7-triacetic acid (DO3A) to bicarbonate was investigated in detail. The response of Eu.DO3A to changes in bicar-bonate concentration was studied in different buffers, and the influence of ionic strength, Ph, and specific ions was determined. The response is found to depend on Ph and ionic strength, but it is not possible to separate contributions from the sensing event from those in the bicarbonate activity. Prior to demonstrating the ability to determine the bicarbonate concentration in bioproduction media and serum, the influence of competing carboxy anions was evaluated. It is established that while competing anions bind stronger to the responsive complex, at the relevant concentration their contribution to the recorded signal is negligible. We are thus able to conclude that Eu.DO3A is a good platform for building a bicarbonate sensor.