论文部分内容阅读
目的:观察健脾清肠散结方及其拆方对肠癌HT-29细胞株增殖作用的影响,并探讨其关键成分及可能的作用机制。方法:将培养的HT-29细胞随机分为:空白对照组,健脾清肠散结方及其拆方健脾益气方、清肠散结方高、低剂量组,共7组。24 h后换无血清培养液,分别加入不同浓度药物(0、2、20、200μg/ml),用噻唑盐比色法(MTT)分析测定各组在不同浓度及不同作用时间点对HT-29细胞生长抑制率。结果:在浓度为2～200μg/ml时,健脾清肠散结方及其拆方对肠癌HT-29的增殖均有一定的抑制作用,呈现浓度依赖和时间依赖性;其中拆方健脾益气方对肠癌HT-29抑制增殖效果与全方一致。结论:健脾清肠散结方对人大肠癌细胞株HT-29细胞增殖有一定的抑制作用,其关键成分可能是健脾益气药。
Objective: To observe the effects of Jianpi Qingchang Sanjie Decoction and its disassembled formulas on the proliferation of colorectal cancer cell line HT-29 and to explore its key components and possible mechanism. Methods: The cultured HT-29 cells were randomly divided into 7 groups: control group, Jianpi Qingchang Sanjie Decoction and its disassembled recipe of Jianpi Yiqi Prescription, Qingchang Sanjie Decoction high and low dose groups. The serum-free medium was changed after 24 h, and different concentrations of drugs (0, 2, 20, 200 μg / ml) were added respectively. MTT assay was used to determine the effect of different concentration and time on the expression of HT- 29 cell growth inhibition rate. Results: When the concentration was 2 ~ 200μg / ml, Jianpi Qingchang Sanjie Decoction and its disassembled prescriptions inhibited the proliferation of HT-29 colon cancer cells in a concentration-dependent and time-dependent manner. Among them, Spleen and Qi on intestinal cancer HT-29 inhibition of proliferation consistent with the whole line. Conclusion: Jianpi Qingchang Sanjie Decoction can inhibit the proliferation of human colorectal cancer cell line HT-29 cells. The key component may be Jianpi Yiqi drug.