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为明确我国东北地区大豆根瘤菌的系统发育地位及主要类群的分布情况,采用BOX-PCR、IGS PCR-RFLP、16SrDNA PCR-RFLP和16S rDNA基因序列分析法对分离自我国东北地区14个地点18个大豆品种的312株大豆根瘤菌及部分参比菌株进行了遗传多样性和系统发育分析。结果表明:供试菌株均为大豆慢生根瘤菌,以Bradyrhizobiumjaponicum为优势种;在IGS PCR-RFLP 72%和16S rDNA PCR-RFLP 76%相似性水平上,供试菌株可分别被分为6和4个群;在BOX-PCR 90%相似性水平上,可将供试菌株分为31个群,表明东北地区慢生大豆根瘤菌具有丰富的遗传多样性。综合考察各种分析结果发现,供试菌株的遗传多样性与其地理来源有一定的相关性,而与大豆品种间相关性不明显。
In order to clarify the phylogeny and distribution of soybean rhizobia in northeastern China, BOX-PCR, IGS PCR-RFLP, 16S rDNA PCR-RFLP and 16S rDNA gene sequence analysis were used to analyze the phylogeny and phylogeny of soybean rhizobium isolated from 14 sites in northeastern China A total of 312 Soybean Rhizobia strains and some reference strains were analyzed for their genetic diversity and phylogenetic relationships. The results showed that all the tested strains were Bradyrhizobium japonicum, and the tested strains were divided into 6 and 16 at the 76% similarity level of PCR-RFLP 72% and 16S rDNA PCR-RFLP, respectively 4 groups. At the 90% similarity level of BOX-PCR, the test strains could be divided into 31 groups, indicating that the rhizobium japonicum was rich in genetic diversity in Northeast China. Comprehensive analysis of various analysis results showed that the genetic diversity of the tested strains and their geographical origin have some relevance, but not obvious correlation with the soybean varieties.