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AIM:To investigate the expression of hypoxia-induciblefactor (HIF)-2α/endothelial PAS domain protein1 (EPAS1)in hepatocellular carcinoma (HCC).METHODS:Expression of HIF-2α/EPAS1 was investigatedimmunohistochemically on paraffin-embedded sections from97 patients with HCC.To further confirm that HIF-2α/EPAS1in HCC tissues also correlated with angiogenesis,a parallelimmunohistchemistry study of vascular endothelial growthfactor (VEGF) was performed on these 97 cases.RESULTS:HIF-2α/EPAS1 could be detected in 50 of 97cases (51.6%),including 19 weakly positive (19.8%),and 31strongly positive (31.1%),the other 47 cases were negative(48.4%).The expression of HIF-2α/EPASlwas significantlycorrelated with tumor size,capsule infiltration,portal veininvasion,and necrosis.A parallel immunohistochemicalanalysis of VEGF demonstrated its positive correlation withcapsule infiltration,portal vein invasion,and HIF-2α/EPAS1overexpression,which supported the correlation of HIF-2α/EPASlup-regulation with tumor angiogenesis.No apparentcorrelation was observed between HIF-2α/EPAS1 and capsularformation,presence of cirrhosis,and histological grade.CONCLUSION:HIF-2α/EPAS1 is expressed in most of HCCwith capsular infiltration and portal vein invasion,whichindicates a possible role of HIF-2α/EPAS1 in HCC metastasis.
AIM: To investigate the expression of hypoxia-inducible factor (HIF) -2α / endothelial PAS domain protein1 (EPAS1) in hepatocellular carcinoma (HCC). METHODS: Expression of HIF-2α / EPAS1 was investigated immunohistochemically on paraffin- embedded sections from 97 patients with HCC .To further confirm that HIF-2α / EPAS1in HCC tissues also correlated with angiogenesis, a parallel immunohistchemistry study of vascular endothelial growth factor (VEGF) was performed on these 97 cases. RESULTS: HIF-2α / EPAS1 could be detected in 50 of 97cases The expression of HIF-2α / EPAS1 was significantlycorrelated with tumor size, capsule infiltration, portal vein invasion, and the other 47 cases were negative (48.4% and necrosis. A parallel immunohistochemicalanalysis of VEGF demonstrated its positive correlation with capsule infiltration, portal vein invasion, and HIF-2α / EPAS1overexpression, which supported the correlation of HIF-2α / EPASlup-regulation w ith tumor angiogenesis. No apparent correlation was observed between HIF-2α / EPAS1 and capsularformation, presence of cirrhosis, and histological grade. CONCLUSION: HIF-2α / EPAS1 is expressed in most of HCC with capsular infiltration and portal vein invasion, whichindications a possible role of HIF-2α / EPAS1 in HCC metastasis.